Since the 16S profiles in the intestine seem pretty different for intracellular and extracellular DNA [33], qPCR is expected to reflect the NGS-specific Neighborhood composition both quantitatively and qualitatively additional closely than circulation cytometry.
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qPCR is relatively simple to accomplish compared to stream cytometry that needs appreciable know-how for reproducible benefits. In truth, move cytometric enumeration of microbial cells was at first limited to pure cultures [31] and even now continues to be hard when done in complex matrices [32].
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